Deregulacja mikroRNA w hipertrofii i kostnieniu
Deregulacja mikroRNA w hipertrofii i kostnieniu Ligamentum Flavum: nowe postępy, wyzwania i potencjalne kierunki
Pathological adjustments within the ligamentum flavum (LF) will be outlined as a means of power progressive aberrations within the nature and construction of ligamentous tissues characterised by elevated thickness, decreased elasticity, native calcification, or aggravated ossification, which can trigger extreme myelopathy, radiculopathy, or each. Hypertrophy of ligamentum flavum (HLF) and ossification of ligamentum flavum (OLF) are clinically frequent entities. Although gathered proof has indicated each genetic and environmental elements may contribute to the initiation and development of HLF/OLF, the particular pathogenesis stays absolutely unclear. MicroRNAs (miRNAs), one of many necessary epigenetic modifications, are brief single-stranded RNA molecules that regulate protein-coding gene expression at posttranscriptional stage, which may disclose the mechanism underlying ailments, determine useful biomarkers, and discover potential therapeutic targets.
Contemplating that miRNAs play a central function in regulating gene expression, we summarized present research from the viewpoint of miRNA-related molecular regulation networks in HLF/OLF. Exploratory research revealed a wide range of miRNA expression profiles and recognized a battery of upregulated and downregulated miRNAs in OLF/HLF sufferers via microarray datasets or transcriptome sequencing. Experimental research validated the roles of particular miRNAs (e.g., miR-132-3p, miR-199b-5p in OLF, miR-155, and miR-21 in HLF) in regulating fibrosis or osteogenesis differentiation of LF cells and associated goal genes or molecular signaling pathways. Lastly, we mentioned the views and challenges of miRNA-based molecular mechanism, diagnostic biomarkers, and therapeutic targets of HLF/OLF.
Rat Stomach 2 Months Total Protein |
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| RT-302-M2 | Zyagen | 1mg | EUR 177 |
Rat Stomach 6 Months Total Protein |
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| RT-302-M6 | Zyagen | 1mg | EUR 177 |
Rat Stomach 1 Month Total Protein |
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| RT-302-M1 | Zyagen | 1mg | EUR 177 |
Mouse CD1 Stomach, whole Total RNA |
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| MR-302 | Zyagen | 0.1mg | EUR 160 |
Mouse C57 Stomach, whole Total RNA |
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| MR-302-C57 | Zyagen | 0.1mg | EUR 180 |
Mouse Balbc Stomach, whole Total RNA |
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| MR-302-BLC | Zyagen | 0.1mg | EUR 180 |
Total RNA - Mouse Normal Tissue: Stomach |
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| R1334248-50 | Biochain | 50 ug | EUR 156 |
Cat Stomach Total RNA |
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| FR-302 | Zyagen | 0.1mg | EUR 195 |
Human Stomach Total RNA |
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| HR-302 | Zyagen | 0.05mg | EUR 172 |
Total RNA - Lupus: Stomach |
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| R1236248Lup-50 | Biochain | 50 ug | EUR 460 |
Sheep Stomach Total RNA |
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| SR-302 | Zyagen | 0.1mg | EUR 160 |
Bovine Stomach Total RNA |
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| BR-302 | Zyagen | 0.1mg | EUR 160 |
Equine Stomach Total RNA |
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| ER-302 | Zyagen | 0.1mg | EUR 195 |
Rabbit Stomach Total RNA |
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| TR-302 | Zyagen | 0.1mg | EUR 160 |
Hamster Stomach Total RNA |
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| AR-302 | Zyagen | 0.1mg | EUR 160 |
MiniPig Stomach Total RNA |
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| NR-302 | Zyagen | 0.1mg | EUR 231 |
Monkey Stomach Total RNA, Rhesus |
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| UR-302 | Zyagen | 0.1mg | EUR 195 |
Monkey Stomach Total RNA, Cynomolgus |
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| KR-302 | Zyagen | 0.1mg | EUR 195 |
Rat Stomach, whole Total RNA |
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| RR-302 | Zyagen | 0.1mg | EUR 160 |
Guinea Pig Stomach Total RNA |
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| GR-302 | Zyagen | 0.1mg | EUR 160 |
Total RNA - Diabetic Disease: Stomach |
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| R1236248Dia-50 | Biochain | 50 ug | EUR 460 |
Tissue, Total RNA, Mouse, Adult Normal, Stomach, BioGenomics |
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| MBS638732-005mg | MyBiosource | 0.05mg | EUR 435 |
Tissue, Total RNA, Mouse, Adult Normal, Stomach, BioGenomics |
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| MBS638732-5x005mg | MyBiosource | 5x0.05mg | EUR 1735 |
Rat WS Stomach, whole Total RNA |
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| RR-302-WS | Zyagen | 0.1mg | EUR 160 |
Total RNA - Rat Normal Tissue: Stomach |
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| R1434248-50 | Biochain | 50 ug | EUR 251 |
Total RNA - Human Tumor Tissue: Stomach |
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| R1235248-50 | Biochain | 50 ug | EUR 480 |
Total RNA - Monkey (Rhesus) Normal Tissue: Stomach |
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| R1534248-50 | Biochain | 50 ug | EUR 229 |
Total RNA - Monkey (Cynomolgus) Normal Tissue: Stomach |
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| R1534248-Cy | Biochain | 50 ug | EUR 229 |
Tissue, Total RNA, Human Adult Normal, Stomach, Corpus of Stomach, BioGenomics |
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| MBS638709-001mg | MyBiosource | 0.01mg | EUR 485 |
Tissue, Total RNA, Human Adult Normal, Stomach, Corpus of Stomach, BioGenomics |
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| MBS638709-5x001mg | MyBiosource | 5x0.01mg | EUR 1960 |
Tissue, Total RNA, Human Adult Normal, Stomach, Fundus of Stomach, BioGenomics |
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| MBS638710-001mg | MyBiosource | 0.01mg | EUR 485 |
Tissue, Total RNA, Human Adult Normal, Stomach, Fundus of Stomach, BioGenomics |
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| MBS638710-5x001mg | MyBiosource | 5x0.01mg | EUR 1960 |
Tissue, Total RNA, Human Adult Normal, Stomach, Cardia of stomach, BioGenomics |
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| MBS638718-001mg | MyBiosource | 0.01mg | EUR 485 |
Tissue, Total RNA, Human Adult Normal, Stomach, Cardia of stomach, BioGenomics |
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| MBS638718-5x001mg | MyBiosource | 5x0.01mg | EUR 1960 |
Tissue, Total RNA, Human Adult Normal, Stomach, Pylorus of stomach, BioGenomics |
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| MBS638566-001mg | MyBiosource | 0.01mg | EUR 485 |
Tissue, Total RNA, Human Adult Normal, Stomach, Pylorus of stomach, BioGenomics |
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| MBS638566-5x001mg | MyBiosource | 5x0.01mg | EUR 1960 |
Total RNA - Human Adult Normal Tissue: Stomach |
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| R1234248-50 | Biochain | 50 ug | EUR 221 |
Mouse CD1 Stomach, whole Total Protein |
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| MT-302 | Zyagen | 1mg | EUR 153 |
Mouse BLC Stomach, whole Total Protein |
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| MT-302-BLC | Zyagen | 1mg | EUR 180 |
Mouse C57 Stomach, whole Total Protein |
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| MT-302-C57 | Zyagen | 1mg | EUR 180 |
Tissue, Total RNA, Human Tumor, Stomach, BioGenomics |
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| MBS638722-005mg | MyBiosource | 0.05mg | EUR 655 |
Tissue, Total RNA, Human Tumor, Stomach, BioGenomics |
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| MBS638722-5x005mg | MyBiosource | 5x0.05mg | EUR 2735 |
Total Protein - Mouse Normal Tissue: Stomach |
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| P1334248 | Biochain | 1 mg | EUR 216 |
Rat Skin 3 Months Total Protein |
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| RT-101-M3 | Zyagen | 1mg | EUR 177 |
Rat Lung 3 Months Total Protein |
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| RT-601-M3 | Zyagen | 1mg | EUR 177 |
Rat Brain 3 Months Total Protein |
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| RT-201-M3 | Zyagen | 1mg | EUR 177 |
Rat Liver 3 Months Total Protein |
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| RT-314-M3 | Zyagen | 1mg | EUR 177 |
Rat Ovary 3 Months Total Protein |
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| RT-406-M3 | Zyagen | 1mg | EUR 177 |
Rat Heart 3 Months Total Protein |
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| RT-801-M3 | Zyagen | 1mg | EUR 177 |
Rat Testis 3 Months Total Protein |
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| RT-401-M3 | Zyagen | 1mg | EUR 177 |
Total RNA - Human Adult Normal Tissue: Stomach: Cardia |
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| R1234250-10 | Biochain | 10 ug | EUR 221 |
Total RNA - Human Adult Normal Tissue: Stomach: Corpus |
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| R1234251-10 | Biochain | 10 ug | EUR 221 |
Total RNA - Human Adult Normal Tissue: Stomach: Fundus |
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| R1234252-10 | Biochain | 10 ug | EUR 221 |
Total RNA - Human Adult Normal Tissue: Stomach: Pylorus |
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| R1234253-10 | Biochain | 10 ug | EUR 221 |
Rat Intestine 3 Months Total Protein |
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| RT-306-M3 | Zyagen | 1mg | EUR 177 |
Tissue, Total RNA, Rat Adult Normal, Stomach, BioGenomics |
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| MBS638527-005mg | MyBiosource | 0.05mg | EUR 505 |
Tissue, Total RNA, Rat Adult Normal, Stomach, BioGenomics |
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| MBS638527-5x005mg | MyBiosource | 5x0.05mg | EUR 2060 |
Tissue, Total RNA, Human Adult Normal, Stomach, BioGenomics |
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| MBS638608-005mg | MyBiosource | 0.05mg | EUR 495 |
Tissue, Total RNA, Human Adult Normal, Stomach, BioGenomics |
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| MBS638608-5x005mg | MyBiosource | 5x0.05mg | EUR 1995 |
Tissue, Total RNA, Human Disease, Lupus, Stomach, BioGenomics |
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| MBS638737-005mg | MyBiosource | 0.05mg | EUR 655 |
Tissue, Total RNA, Human Disease, Lupus, Stomach, BioGenomics |
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| MBS638737-5x005mg | MyBiosource | 5x0.05mg | EUR 2735 |
Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Stomach, BioGenomics |
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| MBS638575-005mg | MyBiosource | 0.05mg | EUR 485 |
Tissue, Total RNA, Monkey (Rhesus) Adult Normal, Stomach, BioGenomics |
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| MBS638575-5x005mg | MyBiosource | 5x0.05mg | EUR 1960 |
Tissue, Total Protein, Mouse Adult Normal, Stomach |
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| MBS657113-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Mouse Adult Normal, Stomach |
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| MBS657113-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Rat S. Muscles 3 Months Total Protein |
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| RT-102-M3 | Zyagen | 1mg | EUR 177 |
Dog Stomach Total Protein |
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| DT-302 | Zyagen | 1mg | EUR 176 |
Cat Stomach Total Protein |
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| FT-302 | Zyagen | 1mg | EUR 176 |
Pig Stomach Total Protein |
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| PT-302 | Zyagen | 1mg | EUR 153 |
Rat Stomach 3 Weeks Total Protein |
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| RT-302-W3 | Zyagen | 1mg | EUR 177 |
Human Stomach Total Protein |
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| HT-302 | Zyagen | 1mg | EUR 176 |
Sheep Stomach Total Protein |
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| ST-302 | Zyagen | 1mg | EUR 153 |
Total RNA - Human Adult Normal Tissue 5 Donor Pool: Stomach |
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| R1234248-P | Biochain | 50 ug | EUR 443 |
Bovine Stomach Total Protein |
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| BT-302 | Zyagen | 1mg | EUR 153 |
Equine Stomach Total protein |
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| ET-302 | Zyagen | 1mg | EUR 176 |
Rabbit Stomach Total Protein |
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| TT-302 | Zyagen | 1mg | EUR 153 |
Hamster Stomach Total Protein |
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| AT-302 | Zyagen | 1mg | EUR 153 |
MiniPig Stomach Total Protein |
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| NT-302 | Zyagen | 1mg | EUR 176 |
Monkey Stomach Total Protein, Rhesus |
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| UT-302 | Zyagen | 1mg | EUR 176 |
Rat Skin 12 Months Total Protein |
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| RT-101-M12 | Zyagen | 1mg | EUR 177 |
Rat Skin 2 Months Total Protein |
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| RT-101-M2 | Zyagen | 1mg | EUR 177 |
Rat Skin 6 Months Total Protein |
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| RT-101-M6 | Zyagen | 1mg | EUR 177 |
Rat Lung 12 Months Total Protein |
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| RT-601-M12 | Zyagen | 1mg | EUR 177 |
Rat Lung 2 Months Total Protein |
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| RT-601-M2 | Zyagen | 1mg | EUR 177 |
Rat Lung 6 Months Total Protein |
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| RT-601-M6 | Zyagen | 1mg | EUR 177 |
Rat Brain 12 Months Total Protein |
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| RT-201-M12 | Zyagen | 1mg | EUR 177 |
Rat Brain 2 Months Total Protein |
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| RT-201-M2 | Zyagen | 1mg | EUR 177 |
Rat Brain 6 Months Total Protein |
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| RT-201-M6 | Zyagen | 1mg | EUR 177 |
Rat Liver 12 Months Total Protein |
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| RT-314-M12 | Zyagen | 1mg | EUR 177 |
Rat Liver 2 Months Total Protein |
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| RT-314-M2 | Zyagen | 1mg | EUR 177 |
Rat Liver 6 Months Total Protein |
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| RT-314-M6 | Zyagen | 1mg | EUR 177 |
Rat Ovary 12 Months Total Protein |
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| RT-406-M12 | Zyagen | 1mg | EUR 177 |
Rat Ovary 2 Months Total Protein |
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| RT-406-M2 | Zyagen | 1mg | EUR 177 |
Rat Ovary 6 Months Total Protein |
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| RT-406-M6 | Zyagen | 1mg | EUR 177 |
Rat Heart 12 Months Total Protein |
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| RT-801-M12 | Zyagen | 1mg | EUR 177 |
Rat Heart 2 Months Total Protein |
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| RT-801-M2 | Zyagen | 1mg | EUR 177 |
Rat Heart 6 Months Total Protein |
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| RT-801-M6 | Zyagen | 1mg | EUR 177 |
Rat Testis 12 Months Total Protein |
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| RT-401-M12 | Zyagen | 1mg | EUR 177 |
Rat Testis 2 Months Total Protein |
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| RT-401-M2 | Zyagen | 1mg | EUR 177 |
Rat Testis 6 Months Total Protein |
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| RT-401-M6 | Zyagen | 1mg | EUR 177 |
Monkey Stomach Total Protein, Cynomolgus |
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| KT-302 | Zyagen | 1mg | EUR 176 |
Rat Stomach E20 Total Protein |
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| RT-302-20 | Zyagen | 1mg | EUR 177 |
Rat Intestine 12 Months Total Protein |
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| RT-306-M12 | Zyagen | 1mg | EUR 177 |
Rat Intestine 2 Months Total Protein |
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| RT-306-M2 | Zyagen | 1mg | EUR 177 |
Rat Intestine 6 Months Total Protein |
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| RT-306-M6 | Zyagen | 1mg | EUR 177 |
Rat Stomach, whole Total Protein |
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| RT-302 | Zyagen | 1mg | EUR 153 |
Guinea Pig Stomach Total Protein |
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| GT-302 | Zyagen | 1mg | EUR 153 |
Human Stomach Lysate, Total Protein |
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| Stomach-004H | Creative BioMart | 100ug | EUR 158.4 |
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Description: Stored at -80 centigrade. |
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Rat S. Muscles 12 Months Total Protein |
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| RT-102-M12 | Zyagen | 1mg | EUR 177 |
Rat S. Muscles 2 Months Total Protein |
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| RT-102-M2 | Zyagen | 1mg | EUR 177 |
Rat S. Muscles 6 Months Total Protein |
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| RT-102-M6 | Zyagen | 1mg | EUR 177 |
Matched Pair - Total RNA - Human Primary Tumor and Normal Tissue: Stomach |
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| R8235248-PP-10 | Biochain | 2x10 ug | EUR 500 |
Rat Stomach 1 Day Total Protein |
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| RT-302-D1 | Zyagen | 1mg | EUR 177 |
Rat Stomach 1 Week Total Protein |
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| RT-302-W1 | Zyagen | 1mg | EUR 177 |
Rat Stomach 2 Weeks Total Protein |
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| RT-302-W2 | Zyagen | 1mg | EUR 177 |
Rat WS Stomach, whole Total Protein |
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| RT-302-WS | Zyagen | 1mg | EUR 153 |
Matched Pair - Total RNA - Human Primary and Metastatic Tumor Tissue: Stomach |
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| R8235248-PM-10 | Biochain | 2x10 ug | EUR 773 |
Total Protein - Human Tumor Tissue: Stomach |
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| P1235248 | Biochain | 1 mg | EUR 357 |
Tissue, Total Protein, Human Tumor, Stomach |
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| MBS657334-1mg | MyBiosource | 1mg | EUR 680 |
Tissue, Total Protein, Human Tumor, Stomach |
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| MBS657334-5x1mg | MyBiosource | 5x1mg | EUR 2835 |
Tissue, Total RNA Matched Pairs, Human Primary Tumor and Normal, Stomach, BioGenomics |
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| MBS638617-2x001mg | MyBiosource | 2x0.01mg | EUR 750 |
Tissue, Total RNA Matched Pairs, Human Primary and Metastasis Tumor, Stomach, BioGenomics |
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| MBS638620-2x001mg | MyBiosource | 2x0.01mg | EUR 985 |
Total Protein - Human Adult Normal Tissue: Stomach |
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| P1234248 | Biochain | 1 mg | EUR 216 |
Tissue, Total Protein, Human Adult Normal, Stomach |
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| MBS657318-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Stomach |
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| MBS657318-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Tissue, Total Protein, Human Disease, Diabetes, Stomach |
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| MBS657368-1mg | MyBiosource | 1mg | EUR 820 |
Tissue, Total Protein, Human Disease, Diabetes, Stomach |
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| MBS657368-5x1mg | MyBiosource | 5x1mg | EUR 3470 |
Total Protein - Human Diabetic Diseased Tissue: Stomach |
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| P1236248Dia | Biochain | 1 mg | EUR 542 |
Total Protein - Human Adult Normal Tissue: Stomach: Cardia |
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| P1234250 | Biochain | 1 mg | EUR 216 |
Total Protein - Human Adult Normal Tissue: Stomach: Corpus |
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| P1234251 | Biochain | 1 mg | EUR 216 |
Total Protein - Human Adult Normal Tissue: Stomach: Fundus |
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| P1234252 | Biochain | 1 mg | EUR 216 |
Tissue, Total Protein, Human Adult Normal, Stomach, Corpus |
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| MBS657372-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Stomach, Corpus |
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| MBS657372-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Tissue, Total Protein, Human Adult Normal, Stomach, Cardia |
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| MBS657595-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Stomach, Cardia |
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| MBS657595-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Tissue, Total Protein, Human Adult Normal, Stomach, Fundus |
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| MBS657608-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Stomach, Fundus |
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| MBS657608-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Total Protein - Human Adult Normal Tissue: Stomach: Pylorus |
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| P1234253 | Biochain | 1 mg | EUR 216 |
Tissue, Total Protein, Human Adult Normal, Stomach, Pylorus |
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| MBS657527-1mg | MyBiosource | 1mg | EUR 565 |
Tissue, Total Protein, Human Adult Normal, Stomach, Pylorus |
|||
| MBS657527-5x1mg | MyBiosource | 5x1mg | EUR 2315 |
Rat Stomach Developmental Total Protein Panel, any 6 stages |
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| RT-302-006D | Zyagen | 6x0.1mg | EUR 998 |
Rat Stomach Developmental Total Protein Panel, any 10 stages |
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| RT-302-010D | Zyagen | 10x0.1mg | EUR 1289 |
Tissue, Total Protein, Matched Pairs, Human Primary Tumor and Normal, Stomach |
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| MBS657350-2x02mg | MyBiosource | 2x0.2mg | EUR 935 |
Dog Stomach RNA |
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| DR-302 | Zyagen | 0.1mg | EUR 195 |
Mouse Stomach, match set of RNA, DNA, Protein |
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| MS-302-RDP | Zyagen | 20µg/20µg/100µg | EUR 422 |
Matched Pair - Total Protein - Human Primary Tumor and Normal Tissue (PP): Stomach |
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| P8235248-PP | Biochain | 0.2 mg x2 | EUR 716 |
Tissue, Total Protein, Matched Pairs, Human Primary Tumor and Metastasis Tumor, Stomach |
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| MBS657320-2x02mg | MyBiosource | 2x0.2mg | EUR 1655 |
Matched Pair - Total Protein - Human Primary Tumor and Metastatic Tumor Tissue (PM): Stomach |
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| P8235248-PM | Biochain | 0.2 mg x2 | EUR 1431 |
Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells |
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| 2-82088 | CHI Scientific | 1 Kit | Ask for price |
Mouse Stomach Genomic DNA |
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| MG-302 | Zyagen | 0.1mg | EUR 177 |
Mouse Stomach Tissue Lysate |
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| IMSSTTL100UG | Innovative research | each | EUR 245 |
|
Description: Mouse Stomach Tissue Lysate |
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Mouse Stomach Tissue Lysate |
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| MBS8420887-01mg | MyBiosource | 0.1mg | EUR 385 |
Mouse Stomach Tissue Lysate |
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| MBS8420887-5x01mg | MyBiosource | 5x0.1mg | EUR 1540 |
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Stomach |
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| MBS640963-1Set | MyBiosource | 1Set | EUR 650 |
Tissue Set, BioAssay, DNA, RNA, Protein, Mouse Adult Normal, Stomach |
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| MBS640963-5x1Set | MyBiosource | 5x1Set | EUR 2970 |
Mouse Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells Growth Medium |
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| 9-32088 | CHI Scientific | 5 x 100 ml | Ask for price |
Mouse Stomach Tissue Preparation Buffer: Normal Stomach Mucosa Epithelial Cells |
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| 9-80202 | CHI Scientific | 1 x 100 ml | Ask for price |
CD-1 Mouse Stomach Cells |
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| ABC-TC3312 | AcceGen | 1 vial | Ask for price |
|
Description: Mouse Primary Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications. |
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BALB/c Mouse Stomach Cells |
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| ABC-TC3105 | AcceGen | 1 vial | Ask for price |
|
Description: Mouse Primary Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Cell Culture Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications. |
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C57BL/6 Mouse Stomach Cells |
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| ABC-TC3247 | AcceGen | 1 vial | Ask for price |
|
Description: Mouse primary stomach cells (MPSTCs) are isolated from tissue of pathogen-free laboratory mice. Mouse primary stomach cells are grown in tissue culture plate pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Cells are negative for bacteria, yeast, fungi, and mycoplasma. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications. |
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Mouse CD1 Stomach, whole cDNA |
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| MD-302 | Zyagen | 30 reactions | EUR 243 |
Mouse BLC Stomach, whole cDNA |
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| MD-302-BLC | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Stomach, whole cDNA |
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| MD-302-C57 | Zyagen | 30 reactions | EUR 280 |
Mouse C57 Stomach Genomic DNA |
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| MG-302-C57 | Zyagen | 0.1mg | EUR 210 |
Mouse Stomach Whole tissue lysate |
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| MAL-1415 | Alpha Diagnostics | 1 mg | EUR 628.8 |
Fenotypowe i epigenetyczne adaptacje limfocytów T CD4 + krwi pępowinowej do otyłości u matki
Pregravid weight problems has been proven to disrupt the event of the offspring’s immune system and improve susceptibility to an infection. Whereas the mechanisms underlying the impression of maternal weight problems on fetal myeloid cells are rising, the results for T cells stay poorly outlined. On this examine, we collected umbilical twine blood samples from infants born to lean moms and moms with weight problems and profiled CD4 T cells utilizing move cytometry and single cell RNA sequencing at resting and following ex vivo polyclonal stimulation. We report that maternal weight problems is related to greater frequencies of reminiscence CD4 T cells suggestive of in vivo activation. Furthermore, single cell RNA sequencing revealed enlargement of an activated subset of reminiscence T cells with maternal weight problems.
Nevertheless, ex vivo stimulation of purified CD4 T cells resulted in poor cytokine responses, suggesting useful defects. These phenotypic and useful aberrations correlated with methylation and chromatin accessibility adjustments in loci related to lymphocyte activation and T cell receptor signaling, suggesting a attainable hyperlink between maternal obesogenic surroundings and fetal immune reprogramming. These observations supply a possible clarification for the elevated susceptibility to microbial an infection in infants born to moms with weight problems.
NIEDOBÓR MELOIDNYCH BIAŁEK PHD POGARSZA ATEROGENEZĘ POPRZEZ APOPTOZĘ MAKROFAGOWĄ I SYGNALIZACJĘ WŁÓKNIKA PARAKRYNOWEGO: Miażdżycowe skutki knockdownu PHD szpiku
Goals: Atherosclerotic plaque hypoxia is detrimental for macrophage perform. Prolyl hydroxylases (PHDs) provoke mobile hypoxic responses, presumably influencing macrophage perform in plaque hypoxia. Thus, we aimed to elucidate the function of myeloid PHDs in atherosclerosis.
Strategies & outcomes: Myeloid particular PHD knockout (PHDko) mice had been obtained through bone marrow transplantation (PHD1ko, PHD3ko) or conditional knockdown via lysozyme M-driven Cre recombinase (PHD2cko). Mice had been fed excessive ldl cholesterol weight loss plan for 6-12 weeks to induce atherosclerosis. Aortic root plaque dimension was considerably augmented 2.6-fold in PHD2cko, and 1.4-fold in PHD3ko in comparison with controls, however was unchanged in PHD1ko mice. Macrophage apoptosis was promoted in PHD2cko and PHD3ko mice in vitro and in vivo, through the HIF1α/BNIP3 axis. Bulk and single cell RNA information of PHD2cko bone-marrow-derived macrophages (BMDM) and plaque macrophages, respectively, confirmed enhanced HIF1α/BNIP3 signaling, which was validated in vitro by siRNA silencing.
Human plaque BNIP3 mRNA was positively related to plaque necrotic core dimension, suggesting related pro-apoptotic results in human. Additional, PHD2cko plaques displayed enhanced fibrosis, whereas macrophage collagen breakdown by matrix metalloproteinases, collagen manufacturing and proliferation had been unaltered. As an alternative, PHD2cko BMDMs enhanced fibroblast collagen secretion in a paracrine method. In silico evaluation of macrophage-fibroblast communication predicted SPP1 (osteopontin) signaling as regulator, which was corroborated by enhanced plaque SPP1 protein in vivo. Elevated SPP1 mRNA expression upon PHD2cko was preferentially noticed in foamy plaque macrophages expressing “triggering receptor expressed on myeloid cells-2” (TREM2hi) evidenced by single-cell RNA, however not in neutrophils. This confirmed enhanced fibrotic signaling by PHD2cko macrophages to fibroblasts, in vitro in addition to in vivo.
Conclusion: Myeloid PHD2cko and PHD3ko enhanced atherosclerotic plaque development and macrophage apoptosis, whereas PHD2cko macrophages additional activated collagen secretion by fibroblasts in vitro, probably through paracrine SPP1 signaling via TREM2hi macrophages.
Translational outlook: This examine reveals that myeloid PHD isoforms PHD2 and PHD3 worsen plaque traits and phenotype, reminiscent of plaque dimension, macrophage accumulation, apoptosis, and collagen accumulation in mice. We present each direct results on macrophages and paracrine results of macrophage PHD2 loss on vessel wall fibroblast populations. Broad spectrum-PHD inhibitors, e.g. Roxadustat, are presently being prescribed to power kidney illness sufferers, who’re already in danger for heart problems. When contemplating this examine and the pro-fibrotic and pro-apoptotic results we report, broad PHD inhibition might subsequently be sub-optimal and extra focused PHD inhibition of PHD1 must be thought of.
Zmiana odpowiedzi interferonu typu I jest związana z subkliniczną miażdżycą tętnic u mężczyzn zakażonych wirusem HIV-1 z supresją wirusologiczną
Background: Given HIV-1 contaminated sufferers have alterations within the sort I interferon (IFN-I) pathway and are additionally at elevated danger of atherosclerosis, we evaluated IFN-I response and subclinical heart problems (CVD) affiliation in HIV-1-infected sufferers.
Strategies: Transcript ranges of IFN-α/β and IFN-stimulated gene (ISG) 56 had been evaluated by RT/Actual-time PCR in PBMCs collected from asymptomatic HIV-1-positive male sufferers at excessive danger of growing CVD (n=34) and wholesome topics (n=21). Stenosis diploma (≥ or < 50%), calcium quantity rating, calcium Agatston rating and myocardial extracellular quantity had been examined by Coronary CT scan. Carotid intima-media thickness (cIMT), Framingham danger rating, atherosclerotic heart problems (ASCVD) and Knowledge Assortment on Antagonistic Results of Anti-HIV Medication (D:A:D) scores had been additionally measured.
Outcomes: Elevated IFN-α, IFN-β and ISG56 ranges had been noticed in all HIV-1-infected males in comparison with wholesome controls (p<0.001 for all genes analysed). HIV-1-infected sufferers with a stenosis diploma ≥50% confirmed greater Framingham danger rating (p=0.019), which was correlated with IFN-β and ISG56 ranges. HIV-1-infected males with enhanced IFN-I ranges and stenosis displayed the next ASCVD calculated danger (p=0.011) and D:A:D (p=0.004). Additionally, there was a pattern towards greater IFN-α and ISG56 mRNA ranges in HIV-1-positive sufferers with an elevated cIMT (p>0.05).
Conclusion: Dysregulation of IFN-I response may take part in the pathogenesis of HIV-associated CVD. This text is protected by copyright. All rights reserved.
AIMP1P2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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ST2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV749656 | ABM | 1.0 ug DNA | EUR 686.4 |
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| LV733636 | ABM | 1.0 ug DNA | Ask for price |
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| LV733690 | ABM | 1.0 ug DNA | Ask for price |
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| LV733744 | ABM | 1.0 ug DNA | Ask for price |
HGF Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV701984 | ABM | 1.0 ug DNA | EUR 540 |
CA8 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708260 | ABM | 1.0 ug DNA | EUR 379.2 |
CA8 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708266 | ABM | 1.0 ug DNA | EUR 379.2 |
CBS Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708344 | ABM | 1.0 ug DNA | EUR 379.2 |
CBS Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708350 | ABM | 1.0 ug DNA | EUR 379.2 |
CGA Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708674 | ABM | 1.0 ug DNA | EUR 379.2 |
CKB Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708758 | ABM | 1.0 ug DNA | EUR 379.2 |
CRK Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708992 | ABM | 1.0 ug DNA | EUR 379.2 |
CRP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV708998 | ABM | 1.0 ug DNA | EUR 379.2 |
CRP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV709004 | ABM | 1.0 ug DNA | EUR 379.2 |
DTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV709472 | ABM | 1.0 ug DNA | EUR 379.2 |
EVL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV709844 | ABM | 1.0 ug DNA | EUR 379.2 |
FTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710252 | ABM | 1.0 ug DNA | EUR 379.2 |
FTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710258 | ABM | 1.0 ug DNA | EUR 379.2 |
FTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710264 | ABM | 1.0 ug DNA | EUR 379.2 |
FTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710270 | ABM | 1.0 ug DNA | EUR 379.2 |
FTL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710276 | ABM | 1.0 ug DNA | EUR 379.2 |
FUZ Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710294 | ABM | 1.0 ug DNA | EUR 379.2 |
GIP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV710408 | ABM | 1.0 ug DNA | EUR 379.2 |
C8A Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV702506 | ABM | 1.0 ug DNA | EUR 540 |
SLN Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV702974 | ABM | 1.0 ug DNA | EUR 540 |
LY9 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV703820 | ABM | 1.0 ug DNA | EUR 540 |
LY9 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV705296 | ABM | 1.0 ug DNA | EUR 540 |
DUT Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV705464 | ABM | 1.0 ug DNA | EUR 540 |
NPL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV705590 | ABM | 1.0 ug DNA | EUR 540 |
BMF Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV705644 | ABM | 1.0 ug DNA | EUR 540 |
FTO Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV706004 | ABM | 1.0 ug DNA | EUR 540 |
MIP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV706508 | ABM | 1.0 ug DNA | EUR 540 |
AEN Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707162 | ABM | 1.0 ug DNA | EUR 379.2 |
AEN Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707168 | ABM | 1.0 ug DNA | EUR 379.2 |
AGK Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707192 | ABM | 1.0 ug DNA | EUR 379.2 |
ALB Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707276 | ABM | 1.0 ug DNA | EUR 379.2 |
ANG Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707330 | ABM | 1.0 ug DNA | EUR 379.2 |
ATM Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707660 | ABM | 1.0 ug DNA | EUR 379.2 |
BID Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707852 | ABM | 1.0 ug DNA | EUR 379.2 |
BMF Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707876 | ABM | 1.0 ug DNA | EUR 379.2 |
BMF Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV707882 | ABM | 1.0 ug DNA | EUR 379.2 |
FTX Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV761758 | ABM | 1.0 ug DNA | EUR 1104 |
CPP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV759286 | ABM | 1.0 ug DNA | EUR 2148 |
CPQ Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV759292 | ABM | 1.0 ug DNA | EUR 686.4 |
TYS Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV754918 | ABM | 1.0 ug DNA | Ask for price |
VCF Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV755578 | ABM | 1.0 ug DNA | Ask for price |
VDI Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV755614 | ABM | 1.0 ug DNA | Ask for price |
WSN Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV755992 | ABM | 1.0 ug DNA | Ask for price |
WT2 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV755998 | ABM | 1.0 ug DNA | Ask for price |
WTS Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV756004 | ABM | 1.0 ug DNA | Ask for price |
WWS Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV756022 | ABM | 1.0 ug DNA | Ask for price |
XCE Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV756046 | ABM | 1.0 ug DNA | Ask for price |
XGR Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV756052 | ABM | 1.0 ug DNA | Ask for price |
XIC Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV756064 | ABM | 1.0 ug DNA | Ask for price |
TDD Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV750298 | ABM | 1.0 ug DNA | Ask for price |
THM Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV750406 | ABM | 1.0 ug DNA | Ask for price |
TOC Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV750682 | ABM | 1.0 ug DNA | Ask for price |
PB1 Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV811124 | ABM | 1.0 ug DNA | EUR 379.2 |
GBL Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV811202 | ABM | 1.0 ug DNA | EUR 379.2 |
NLP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV811940 | ABM | 1.0 ug DNA | EUR 379.2 |
DCI Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV812306 | ABM | 1.0 ug DNA | EUR 379.2 |
RIP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV812624 | ABM | 1.0 ug DNA | EUR 379.2 |
CFP Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV791318 | ABM | 1.0 ug DNA | EUR 379.2 |
ECD Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV791504 | ABM | 1.0 ug DNA | EUR 379.2 |
HBB Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV791768 | ABM | 1.0 ug DNA | EUR 379.2 |
NLK Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV792182 | ABM | 1.0 ug DNA | EUR 379.2 |
NMB Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV792188 | ABM | 1.0 ug DNA | EUR 379.2 |
NMB Lentiviral Vector (Human) (EF1a) (pLenti-GIII-EF1a) |
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| LV792194 | ABM | 1.0 ug DNA | EUR 379.2 |
