ILF3-AS1 promuje tlenową glikolizę

• Pawel
• 17 maja, 202130 kwietnia, 2021
• 0

ILF3-AS1 promuje tlenową glikolizę i proliferację komórek czerniaka poprzez regulację szlaku miR-493-5p / PDK1 

Background: To analyze the function of ILF3-AS1 in regulating the survival of melanoma and its molecular mechanism.

Strategies: The relative expression stage of ILF3-AS1 in melanoma was assessed by qPCR. The impact of ILF3-AS1 and PDK1 on the cell viability was examined by MTT assay. Glucose uptake colorimetric assay, lactate assay, the measurements of extracellular acidification charge (ECAR) and Oxygen consumption charge (OCR) have been carried out to check the impact of ILF3-AS1 and PDK1 on the mobile glycolysis. Luciferase assay was performed to detect the interactions of ILF3-AS1, miR-493-5p and PDK1. RNA immunoprecipitation chip (RIP) assay was used to detect the enrichments of ILF3-AS1 and miR-493-5p within the complicated. Protein stage of PDK1 was detected by western blot evaluation.

Outcomes: qPCR revealed that ILF3-AS1 was upregulated in human melanoma cell strains. MTT assay confirmed that ILF3-AS1 knockdown blunted cell proliferation, which was rescued by the overexpression of PDK1. Glucose uptake colorimetric assay, lactate assay, the measurements of ECAR and OCR indicated that ILF3-AS1 promoted glycolysis via PDK1. Western blotting outcomes confirmed that ILF3-AS1 overexpression promoted PDK1 expression, which was prevented by miR-493-5p overexpression in SK-MEL-1 cells.

Conclusions: ILF3-AS1 promotes the cardio glycolysis and survival of melanoma cells involving miR-493-5p/PDK1 pathway.

Retinoblastoma Protein 1 (RB1) Antibody
20-abx126458	Abbexa	EUR 493.20 EUR 710.40 EUR 218.40 EUR 376.80	100 ul 200 ul 20 ul 50 ul

Retinoblastoma Protein 1 (RB1) Antibody
abx011911-100ul	Abbexa	100 ul	EUR 493.2

Retinoblastoma Protein 1 (RB1) Antibody
abx032759-400ul	Abbexa	400 ul	EUR 627.6

Retinoblastoma Protein 1 (RB1) Antibody
abx032759-80l	Abbexa	80 µl	EUR 343.2

Retinoblastoma Protein 1 (RB1) Antibody
abx034004-400ul	Abbexa	400 ul	EUR 627.6

Retinoblastoma Protein 1 (RB1) Antibody
abx034004-80l	Abbexa	80 µl	EUR 343.2

Retinoblastoma Protein 1 (RB1) Antibody
abx037361-100ug	Abbexa	100 ug	EUR 469.2

Retinoblastoma Protein 1 (RB1) Antibody
20-abx320518	Abbexa	EUR 360.00 EUR 292.80	100 ul 50 ul

Retinoblastoma Protein 1 (RB1) Antibody
20-abx329342	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx329620	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
abx331681-100ul	Abbexa	100 ul	EUR 510

Retinoblastoma Protein 1 (RB1) Antibody
abx332050-100ul	Abbexa	100 ul	EUR 510

Retinoblastoma Protein 1 (RB1) Antibody
abx332098-100ul	Abbexa	100 ul	EUR 510

Retinoblastoma Protein 1 (RB1) Antibody
abx332214-100ul	Abbexa	100 ul	EUR 510

Retinoblastoma Protein 1 (RB1) Antibody
20-abx326789	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx326790	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx326791	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx328550	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx328752	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Antibody
20-abx214314	Abbexa	EUR 493.20 EUR 360.00	100 ul 50 ul

Retinoblastoma Protein 1 (RB1) Antibody
abx237141-100ug	Abbexa	100 ug	EUR 610.8

Retinoblastoma Protein 1 (RB1) Antibody
abx237142-100ug	Abbexa	100 ug	EUR 661.2

Retinoblastoma Antibody / Rb1
RQ5140	NSJ Bioreagents	100ul	EUR 419

Retinoblastoma Antibody / Rb1
RQ5141	NSJ Bioreagents	100ul	EUR 419

Retinoblastoma Antibody / Rb1
F54327-0.05ML	NSJ Bioreagents	0.05 ml	EUR 165

Retinoblastoma Antibody / Rb1
F54327-0.2ML	NSJ Bioreagents	0.2 ml	EUR 379

Retinoblastoma Antibody / Rb1
V3218-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

Retinoblastoma Antibody / Rb1
V3218-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

Retinoblastoma Antibody / Rb1
V3218SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein.

Retinoblastoma Antibody / Rb1
V3438-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3438-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3438SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3439-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3439-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3439SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3578-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3578-20UG	NSJ Bioreagents	20 ug	EUR 219
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3578IHC-7ML	NSJ Bioreagents	7 ml	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Antibody / Rb1
V3578SAF-100UG	NSJ Bioreagents	100 ug	EUR 499
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Retinoblastoma Protein 1 (RB1) Protein
20-abx260454	Abbexa	EUR 276.00 EUR 2798.40 EUR 393.60	10 ug 1 mg 50 ug

Recombinant Retinoblastoma Protein 1 (RB1)
4-RPB211Hu01	Cloud-Clone	EUR 507.07 EUR 259.20 EUR 1571.52 EUR 603.84 EUR 1087.68 EUR 415.20 EUR 3748.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli

Recombinant Retinoblastoma Protein 1 (RB1)
4-RPB211Hu02	Cloud-Clone	EUR 507.07 EUR 259.20 EUR 1571.52 EUR 603.84 EUR 1087.68 EUR 415.20 EUR 3748.80	100 ug 10ug 1 mg 200 ug 500 ug 50ug 5 mg
Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli

Retinoblastoma Protein 1 (Rb1) Antibody Pair
abx117561-1pair5x96wellplates	Abbexa	1 pair (5x96 well plates)	EUR 1212

Retinoblastoma Protein 1 (RB1) Antibody (Biotin)
20-abx272104	Abbexa	EUR 543.60 EUR 292.80 EUR 1579.20 EUR 744.00 EUR 410.40	100 ug 10 ug 1 mg 200 ug 50 ug

Rat Retinoblastoma-associated protein (Rb1)
1-CSB-RP155294r(A3)	Cusabio	EUR 606.00 EUR 318.00 EUR 2192.40 EUR 919.20 EUR 1461.60 EUR 402.00	100ug 10ug 1MG 200ug 500ug 50ug
Description: Recombinant Rat Retinoblastoma-associated protein(Rb1),partial expressed in E.coli

Human Retinoblastoma Protein 1 (RB1) Protein
20-abx068874	Abbexa	EUR 710.40 EUR 309.60 EUR 2131.20 EUR 844.80 EUR 526.80	100 ug 10 ug 1 mg 200 ug 50 ug

Human Retinoblastoma Protein 1 (RB1) Protein
20-abx068875	Abbexa	EUR 710.40 EUR 309.60 EUR 2131.20 EUR 844.80 EUR 526.80	100 ug 10 ug 1 mg 200 ug 50 ug

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human)
4-PAB211Hu01	Cloud-Clone	EUR 286.80 EUR 2869.20 EUR 717.60 EUR 358.80 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1)

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human)
4-PAB211Hu02	Cloud-Clone	EUR 286.80 EUR 2869.20 EUR 717.60 EUR 358.80 EUR 253.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1)

Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
20-abx125454	Abbexa	EUR 710.40 EUR 1028.40 EUR 493.20	100 ul 200 ul 50 ul

Retinoblastoma Protein 1 Phospho-Ser612 (RB1 pS612) Antibody
20-abx323083	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody
abx333137-100ul	Abbexa	100 ul	EUR 560.4

Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody
abx333182-100ul	Abbexa	100 ul	EUR 560.4

Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody
abx333183-100ul	Abbexa	100 ul	EUR 560.4

Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
abx333270-100ul	Abbexa	100 ul	EUR 560.4

Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody
20-abx329208	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser811 (RB1 pS811) Antibody
20-abx329209	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody
20-abx329210	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser249 (RB1 pS249) Antibody
20-abx326387	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser788 (RB1 pS788) Antibody
20-abx326427	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Thr821 (RB1 pT821) Antibody
20-abx326768	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody
20-abx328301	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
20-abx328302	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 Phospho-Ser608 (RB1 pS608) Antibody
20-abx328753	Abbexa	EUR 376.80 EUR 292.80	100 ug 50 ug

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC
4-PAB211Hu01-APC	Cloud-Clone	EUR 399.60 EUR 3735.60 EUR 1046.40 EUR 507.60 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated
4-PAB211Hu01-Biotin	Cloud-Clone	EUR 363.60 EUR 2809.20 EUR 836.40 EUR 442.80 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3
4-PAB211Hu01-Cy3	Cloud-Clone	EUR 483.60 EUR 4930.80 EUR 1345.20 EUR 627.60 EUR 294.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC
4-PAB211Hu01-FITC	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP
4-PAB211Hu01-HRP	Cloud-Clone	EUR 366.00 EUR 3256.80 EUR 926.40 EUR 459.60 EUR 243.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE
4-PAB211Hu01-PE	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC
4-PAB211Hu02-APC	Cloud-Clone	EUR 399.60 EUR 3735.60 EUR 1046.40 EUR 507.60 EUR 258.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated
4-PAB211Hu02-Biotin	Cloud-Clone	EUR 363.60 EUR 2809.20 EUR 836.40 EUR 442.80 EUR 259.20	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3
4-PAB211Hu02-Cy3	Cloud-Clone	EUR 483.60 EUR 4930.80 EUR 1345.20 EUR 627.60 EUR 294.00	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC
4-PAB211Hu02-FITC	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP
4-PAB211Hu02-HRP	Cloud-Clone	EUR 366.00 EUR 3256.80 EUR 926.40 EUR 459.60 EUR 243.60	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP.

Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE
4-PAB211Hu02-PE	Cloud-Clone	EUR 344.40 EUR 3012.00 EUR 860.40 EUR 430.80 EUR 230.40	100ul 10ml 1ml 200ul 20ul
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE.

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
20-abx152929	Abbexa	EUR 8534.40 EUR 4550.40 EUR 1054.80	10 × 96 tests 5 × 96 tests 96 tests

Monkey Retinoblastoma Protein 1 (RB1) ELISA Kit
abx358739-96tests	Abbexa	96 tests	EUR 990

Rabbit Retinoblastoma Protein 1 (RB1) ELISA Kit
abx363643-96tests	Abbexa	96 tests	EUR 990

Sheep Retinoblastoma Protein 1 (RB1) ELISA Kit
abx364326-96tests	Abbexa	96 tests	EUR 1111.2

Pig Retinoblastoma Protein 1 (RB1) ELISA Kit
abx360817-96tests	Abbexa	96 tests	EUR 990

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
abx253095-96tests	Abbexa	96 tests	EUR 848.4

Chicken Retinoblastoma Protein 1 (RB1) ELISA Kit
abx355616-96tests	Abbexa	96 tests	EUR 990

Human Retinoblastoma Protein 1 (RB1) CLIA Kit
20-abx492490	Abbexa	EUR 9567.60 EUR 5095.20 EUR 1177.20	10 × 96 tests 5 × 96 tests 96 tests

Human RB1(Retinoblastoma Protein 1) ELISA Kit
EH3703	FN Test	96T	EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-10x96wellstestplate	Cloud-Clone	10x96-wells test plate	EUR 5402.92
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-1x48wellstestplate	Cloud-Clone	1x48-wells test plate	EUR 550.13
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-1x96wellstestplate	Cloud-Clone	1x96-wells test plate	EUR 734.46
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-5x96wellstestplate	Cloud-Clone	5x96-wells test plate	EUR 2945.08
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.

Human Retinoblastoma Protein 1 (RB1) ELISA Kit
4-SEB211Hu	Cloud-Clone	EUR 5463.60 EUR 2886.00 EUR 735.60	10 plates of 96 wells 5 plates of 96 wells 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Retinoblastoma Protein 1 (RB1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rozpoznanie H3K9me1 przez czynnik metylacji DNA SHH2 kierowany przez kukurydzę RNA 

RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has intensive crosstalk with histone modifications. Right here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural research reveal that H3K9me1 recognition is achieved by recognition of the methyl group through a basic fragrant cage and hydrogen-bonding and salt bridge interactions with the free protons of the mono-methyllysine.

The di- and tri-methylation states disrupt the polar interactions, lowering the binding affinity. Our examine reveals a mono-methyllysine recognition mechanism which doubtlessly hyperlinks RdDM to H3K9me1 in maize. This text is protected by copyright. All rights reserved.

Działanie kontrolujące jakość rybosomów nasila syntezę białek wirusa krowianki podczas infekcji

 Viral an infection each prompts stress signaling pathways and redistributes ribosomes away from host mRNAs to translate viral mRNAs. The intricacies of this ribosome shuffle from host to viral mRNAs are poorly understood. Right here, we uncover a job for the ribosome-associated high quality management (RQC) issue ZNF598 throughout vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 (RPS20) and eS10 (RPS10), initiating RQC-dependent nascent chain degradation and ribosome recycling. We present that vaccinia an infection enhances uS10 ubiquitylation, indicating an elevated burden on RQC pathways throughout viral propagation.

According to an elevated RQC demand, we exhibit that vaccinia virus replication is impaired in cells that both lack ZNF598 or categorical a ubiquitylation-deficient model of uS10. Utilizing SILAC-based proteomics and concurrent RNA-seq evaluation, we decide that translation, however not transcription of vaccinia virus mRNAs is compromised in cells with poor RQC exercise. Moreover, vaccinia virus an infection reduces mobile RQC exercise, suggesting that co-option of ZNF598 by vaccinia virus performs a crucial function in translational reprogramming that’s wanted for optimum viral propagation.

Guzy jąder zarodkowych powstają przy braku różnicowania specyficznego dla płci 

In response to indicators from the embryonic testis, the germ cell intrinsic issue NANOS2 coordinates a transcriptional program crucial for the differentiation of pluripotent-like primordial germ cells towards a unipotent spermatogonial stem cell destiny. Rising proof signifies that genetic danger components contribute to testicular germ cell tumor initiation by disrupting sex-specific differentiation. Right here, utilizing the 129.MOLF-Chr19 mouse mannequin of testicular teratomas and a NANOS2 reporter allele, we report that the developmental phenotypes required for tumorigenesis, together with failure to enter mitotic arrest, retention of pluripotency and delayed sex-specific differentiation, have been unique to a subpopulation of germ cells failing to specific NANOS2. Single-cell RNA sequencing revealed that embryonic day 15.5 NANOS2-deficient germ cells and embryonal carcinoma cells developed a transcriptional profile enriched for MYC signaling, NODAL signaling and primed pluripotency.

Furthermore, lineage-tracing experiments demonstrated that embryonal carcinoma cells arose completely from germ cells failing to specific NANOS2. Our outcomes point out that NANOS2 is the nexus via which a number of genetic danger components affect tumor susceptibility. We suggest that, within the absence of intercourse specification, indicators native to the growing testis drive germ cell transformation.

Indukowane przez BMP4 różnicowanie komórek macierzystych grzebienia mieszków włosowych ludzkiego włosa w melanocyty prekursorowe z wybrzuszenia mieszków włosowych 

Background: Vitiligo is a pores and skin depigmentation dysfunction, for which, repigmentation remedy with mixed follicular unit extraction (FUE) graft and narrowband ultraviolet B (NBUVB) is taken into account superior to micro-punch graft remedy. BMP4 can induce MITF expression in Neural crest stem cells (NCSCs), and α-MSH subsequently promotes the differentiation of MITF-expressing cells alongside the melanocyte lineage.

Goal: To analyze why FUE grafting is superior to epidermal mini grafting in selling hair follicles (HF) melanocyte cell survival and longevity, we deliberate the in vitro experiments HF bulge NCSCs differentiate into melanocyte precursors underneath the co-treatment of BMP4 and α-MSH.

Strategies: Cells that migrated from the HF bulge of scalp have been cultured and assessed utilizing immunofluorescence. Transcriptome evaluation was carried out on RNA sequencing outcomes.

Outcomes: Fundamental fibroblast progress issue promotes the proliferation and survival of NCSCs, with spontaneous differentiation into SOX10+/SOX2+ glial progenitors, however not into SOX10+/MITF+ precursor melanocytes. Each BMP4 and α-MSH promoted the differentiation into MITF-expressing cells. RNA sequencing revealed a downregulation in neuregulin-1 (NRG1) and sermaphorin 3C (SEMA3C), and upregulation in WNT10A. Moreover, FUE grafting had a supply of reservoir melanocytes superior to mini- grafting in remedy for vitiligo.

Conclusion: We obtained SOX10+/MITF+ precursor melanocytes via an induction of differentiation alongside the melanocyte lineage by BMP4 and α-MSH. Based on the RNA sequencing outcomes that NRG1 and SEMA3C have been downregulated and WNT10A was upregulated, we postulated that HF NCSCs differentiated into melanocyte by co-treatment of BMP4 and α-MSH. Total, FUE grafting is a extra sturdy and substitutive remedy possibility for vitiligo.

Analiza sieci ważonej koekspresji genów genów centralnych w gruczolakoraku płuc 

Lung adenocarcinoma (LUAD) is a tumor with excessive incidence. This examine aimed to determine the central genes of LUAD. LUAD have been analyzed by weighted gene co-expression community (WGCNA), and differentially expressed genes (DEGs) have been recognized. Samples have been obtained from The Most cancers Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases and included 515 LUAD samples and 347 regular samples. The WGCNA algorithm generated a complete of 10 modules. The highest 2 modules (MEturquoise and MEblue) with the best correlation to LUAD have been chosen.

Ten Hub genes (IL6, CDH1, PECAM1, SPP1, THBS1, HGF, SNCA, CDH5, CAV1, and DLC1) have been screened within the intersecting genes of DEGs and WGCNA (MEturquoise and MEblue). Solely SPP1 was correlated with LUAD poor survival, indicating that SPP1 could also be a key Hub gene for LUAD. The competing endogenous RNA (ceRNA) community was constructed to investigate the regulatory relationship of Hub genes, and SPP1 could also be instantly regulated by four microRNAs (miRNAs) and not directly regulated by 49 lengthy noncoding RNAs (lncRNAs).