• May 17, 2021
chikwadrat

ILF3-AS1 promuje tlenową glikolizę

ILF3-AS1 promuje tlenową glikolizę i proliferację komórek czerniaka poprzez regulację szlaku miR-493-5p / PDK1 

Background: To analyze the function of ILF3-AS1 in regulating the survival of melanoma and its molecular mechanism.

Strategies: The relative expression stage of ILF3-AS1 in melanoma was assessed by qPCR. The impact of ILF3-AS1 and PDK1 on the cell viability was examined by MTT assay. Glucose uptake colorimetric assay, lactate assay, the measurements of extracellular acidification charge (ECAR) and Oxygen consumption charge (OCR) have been carried out to check the impact of ILF3-AS1 and PDK1 on the mobile glycolysis. Luciferase assay was performed to detect the interactions of ILF3-AS1, miR-493-5p and PDK1. RNA immunoprecipitation chip (RIP) assay was used to detect the enrichments of ILF3-AS1 and miR-493-5p within the complicated. Protein stage of PDK1 was detected by western blot evaluation.

Outcomes: qPCR revealed that ILF3-AS1 was upregulated in human melanoma cell strains. MTT assay confirmed that ILF3-AS1 knockdown blunted cell proliferation, which was rescued by the overexpression of PDK1. Glucose uptake colorimetric assay, lactate assay, the measurements of ECAR and OCR indicated that ILF3-AS1 promoted glycolysis via PDK1. Western blotting outcomes confirmed that ILF3-AS1 overexpression promoted PDK1 expression, which was prevented by miR-493-5p overexpression in SK-MEL-1 cells.

Conclusions: ILF3-AS1 promotes the cardio glycolysis and survival of melanoma cells involving miR-493-5p/PDK1 pathway.

chikwadrat
chikwadrat
Retinoblastoma Protein 1 (RB1) Antibody
20-abx123188
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx126458
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx037361-100ug 100 ug
EUR 391
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx136094
  • EUR 411.00
  • EUR 592.00
  • 100 ul
  • 200 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx141027
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx141070
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx141167
  • EUR 356.00
  • EUR 537.00
  • EUR 217.00
  • 100 ul
  • 200 ul
  • 30 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx011911-100ul 100 ul
EUR 411
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx032759-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx032759-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx034004-400ul 400 ul
EUR 523
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx034004-80l 80 µl
EUR 286
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx320518
  • EUR 300.00
  • EUR 244.00
  • 100 ul
  • 50 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx237141-100ug 100 ug
EUR 509
  • Shipped within 5-12 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx237142-100ug 100 ug
EUR 551
  • Shipped within 5-12 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx329342
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx329620
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx331681-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx332050-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx332098-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
abx332214-100ul 100 ul
EUR 425
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx326789
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx326790
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx326791
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx328550
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx328752
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody
20-abx001728
  • EUR 411.00
  • EUR 592.00
  • EUR 182.00
  • EUR 314.00
  • 100 ul
  • 200 ul
  • 20 ul
  • 50 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Protein
20-abx260454
  • EUR 230.00
  • EUR 2332.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug
  • Shipped within 5-10 working days.
Recombinant Retinoblastoma Protein 1 (RB1)
4-RPB211Hu01
  • EUR 422.56
  • EUR 216.00
  • EUR 1309.60
  • EUR 503.20
  • EUR 906.40
  • EUR 346.00
  • EUR 3124.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P06400
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 21.4kDa
  • Isoelectric Point: Inquire
Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli
Recombinant Retinoblastoma Protein 1 (RB1)
4-RPB211Hu02
  • EUR 422.56
  • EUR 216.00
  • EUR 1309.60
  • EUR 503.20
  • EUR 906.40
  • EUR 346.00
  • EUR 3124.00
  • 100 ug
  • 10ug
  • 1 mg
  • 200 ug
  • 500 ug
  • 50ug
  • 5 mg
  • Uniprot ID: P06400
  • Buffer composition: PBS, pH 7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
  • Form: Freeze-dried powder
  • Predicted Molecular Mass (KD): 49.2kDa
  • Isoelectric Point: 7.7
Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli
Retinoblastoma Protein 1 (Rb1) Antibody Pair
abx117561-1pair5x96wellplates 1 pair (5x96 well plates)
EUR 1010
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Antibody (Biotin)
20-abx272104
  • EUR 453.00
  • EUR 244.00
  • EUR 1316.00
  • EUR 620.00
  • EUR 342.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.
Human Retinoblastoma Protein 1 (RB1) Protein
20-abx068874
  • EUR 592.00
  • EUR 258.00
  • EUR 1776.00
  • EUR 704.00
  • EUR 439.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Human Retinoblastoma Protein 1 (RB1) Protein
20-abx068875
  • EUR 592.00
  • EUR 258.00
  • EUR 1776.00
  • EUR 704.00
  • EUR 439.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human)
4-PAB211Hu01
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1)
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human)
4-PAB211Hu02
  • EUR 239.00
  • EUR 2391.00
  • EUR 598.00
  • EUR 299.00
  • EUR 211.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1)
Rat Retinoblastoma-associated protein (Rb1)
1-CSB-RP155294r(A3)
  • EUR 505.00
  • EUR 265.00
  • EUR 1827.00
  • EUR 766.00
  • EUR 1218.00
  • EUR 335.00
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 26.6 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Rat Retinoblastoma-associated protein(Rb1),partial expressed in E.coli
Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
20-abx125454
  • EUR 592.00
  • EUR 857.00
  • EUR 411.00
  • 100 ul
  • 200 ul
  • 50 ul
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody
20-abx329208
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser811 (RB1 pS811) Antibody
20-abx329209
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody
20-abx329210
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser249 (RB1 pS249) Antibody
20-abx326387
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser788 (RB1 pS788) Antibody
20-abx326427
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Thr821 (RB1 pT821) Antibody
20-abx326768
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody
20-abx328301
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
20-abx328302
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser608 (RB1 pS608) Antibody
20-abx328753
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser612 (RB1 pS612) Antibody
20-abx323083
  • EUR 314.00
  • EUR 244.00
  • 100 ug
  • 50 ug
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody
abx333137-100ul 100 ul
EUR 467
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody
abx333182-100ul 100 ul
EUR 467
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody
abx333183-100ul 100 ul
EUR 467
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody
abx333270-100ul 100 ul
EUR 467
  • Shipped within 5-10 working days.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC
4-PAB211Hu01-APC
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated
4-PAB211Hu01-Biotin
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3
4-PAB211Hu01-Cy3
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC
4-PAB211Hu01-FITC
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP
4-PAB211Hu01-HRP
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE
4-PAB211Hu01-PE
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC
4-PAB211Hu02-APC
  • EUR 333.00
  • EUR 3113.00
  • EUR 872.00
  • EUR 423.00
  • EUR 215.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated
4-PAB211Hu02-Biotin
  • EUR 303.00
  • EUR 2341.00
  • EUR 697.00
  • EUR 369.00
  • EUR 216.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3
4-PAB211Hu02-Cy3
  • EUR 403.00
  • EUR 4109.00
  • EUR 1121.00
  • EUR 523.00
  • EUR 245.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC
4-PAB211Hu02-FITC
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP
4-PAB211Hu02-HRP
  • EUR 305.00
  • EUR 2714.00
  • EUR 772.00
  • EUR 383.00
  • EUR 203.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE
4-PAB211Hu02-PE
  • EUR 287.00
  • EUR 2510.00
  • EUR 717.00
  • EUR 359.00
  • EUR 192.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
20-abx152929
  • EUR 7112.00
  • EUR 3792.00
  • EUR 879.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
abx253095-96tests 96 tests
EUR 707
  • Shipped within 5-12 working days.
Human RB1(Retinoblastoma Protein 1) ELISA Kit
EH3703 96T
EUR 524.1
  • Detection range: 0.156-10 ng/ml
  • Uniprot ID: P06400
  • Alias: RB1/OSRC/p105-Rb/pp110/OSRC/osteosarcoma/p105-Rb/pp110/pRb/Rb/RB/retinoblastoma 1/retinoblastoma suspectibility protein/retinoblastoma-associated protein
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml
Pig Retinoblastoma Protein 1 (RB1) ELISA Kit
abx360817-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Monkey Retinoblastoma Protein 1 (RB1) ELISA Kit
abx358739-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Chicken Retinoblastoma Protein 1 (RB1) ELISA Kit
abx355616-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Rabbit Retinoblastoma Protein 1 (RB1) ELISA Kit
abx363643-96tests 96 tests
EUR 825
  • Shipped within 5-12 working days.
Sheep Retinoblastoma Protein 1 (RB1) ELISA Kit
abx364326-96tests 96 tests
EUR 926
  • Shipped within 5-12 working days.
Human Retinoblastoma Protein 1 (RB1) CLIA Kit
20-abx492490
  • EUR 7973.00
  • EUR 4246.00
  • EUR 981.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.
Human Retinoblastoma Protein 1(RB1)ELISA Kit
QY-E01519 96T
EUR 361
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-10x96wellstestplate 10x96-wells test plate
EUR 4502.43
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Retinoblastoma Protein 1 (RB1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-1x48wellstestplate 1x48-wells test plate
EUR 458.44
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Retinoblastoma Protein 1 (RB1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-1x96wellstestplate 1x96-wells test plate
EUR 612.05
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Retinoblastoma Protein 1 (RB1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
SEB211Hu-5x96wellstestplate 5x96-wells test plate
EUR 2454.23
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Retinoblastoma Protein 1 (RB1) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids.
Human Retinoblastoma Protein 1 (RB1) ELISA Kit
4-SEB211Hu
  • EUR 4553.00
  • EUR 2405.00
  • EUR 613.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Retinoblastoma Protein 1 elisa. Alternative names of the recognized antigen: OSRC
  • PRB
  • p105-Rb
  • pp110
  • Including Osteosarcoma
  • Retinoblastoma-associated protein
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Retinoblastoma Protein 1 (RB1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC-Cy7
4-PAB211Hu01-APC-Cy7
  • EUR 547.00
  • EUR 6106.00
  • EUR 1624.00
  • EUR 727.00
  • EUR 310.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Ile753~Lys928)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC-Cy7.
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC-Cy7
4-PAB211Hu02-APC-Cy7
  • EUR 547.00
  • EUR 6106.00
  • EUR 1624.00
  • EUR 727.00
  • EUR 310.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: RB1 (Gln639~Thr778)
  • Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC-Cy7.
ELISA kit for Human RB1 (Retinoblastoma Protein 1)
E-EL-H1932 1 plate of 96 wells
EUR 534
  • Gentaur's RB1 ELISA kit utilizes the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human RB1. Standards or samples are added to the micro ELISA plate wells and combined with the
  • Show more
Description: A sandwich ELISA kit for quantitative measurement of Human RB1 (Retinoblastoma Protein 1) in samples from Serum, Plasma, Cell supernatant
ELISA kit for Human RB1 (Retinoblastoma Protein 1)
ELK4967 1 plate of 96 wells
EUR 432
  • The microtiter plate provided in this kit has been pre-coated with an antibody specific to Retinoblastoma Protein 1 (RB1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ret
  • Show more
Description: A sandwich ELISA kit for detection of Retinoblastoma Protein 1 from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
RB1 Retinoblastoma Associated Protein Human Recombinant Protein
PROTP06400 Regular: 50ug
EUR 317
Description: Retinoblastoma Human Recombinant fused with 6X His tag produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 16.5 kDa.;The Retinoblastoma is purified by proprietary chromatographic techniques.
Chicken Retinoblastoma- associated protein, RB1 ELISA KIT
ELI-14932c 96 Tests
EUR 928
Mouse Retinoblastoma- associated protein, Rb1 ELISA KIT
ELI-18205m 96 Tests
EUR 865
Human Retinoblastoma- associated protein, RB1 ELISA KIT
ELI-39173h 96 Tests
EUR 824
Polyclonal RB1 / Retinoblastoma / RB Antibody (N-Terminus)
APR02413G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RB1 / Retinoblastoma / RB (N-Terminus). This antibody is tested and proven to work in the following applications:
Polyclonal RB1 / Retinoblastoma / RB Antibody (aa781-830)
APR02916G 0.05ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human RB1 / Retinoblastoma / RB (aa781-830). This antibody is tested and proven to work in the following applications:
Anti-Phospho-Retinoblastoma (S780) Rabbit Monoclonal Antibody
M00039-1 100ug/vial
EUR 397
Description: Rabbit Monoclonal Phospho-Retinoblastoma (S780) Antibody. Validated in IHC, WB and tested in Human.

Rozpoznanie H3K9me1 przez czynnik metylacji DNA SHH2 kierowany przez kukurydzę RNA 

RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has intensive crosstalk with histone modifications. Right here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural research reveal that H3K9me1 recognition is achieved by recognition of the methyl group through a basic fragrant cage and hydrogen-bonding and salt bridge interactions with the free protons of the mono-methyllysine.

The di- and tri-methylation states disrupt the polar interactions, lowering the binding affinity. Our examine reveals a mono-methyllysine recognition mechanism which doubtlessly hyperlinks RdDM to H3K9me1 in maize. This text is protected by copyright. All rights reserved.

Działanie kontrolujące jakość rybosomów nasila syntezę białek wirusa krowianki podczas infekcji

 Viral an infection each prompts stress signaling pathways and redistributes ribosomes away from host mRNAs to translate viral mRNAs. The intricacies of this ribosome shuffle from host to viral mRNAs are poorly understood. Right here, we uncover a job for the ribosome-associated high quality management (RQC) issue ZNF598 throughout vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 (RPS20) and eS10 (RPS10), initiating RQC-dependent nascent chain degradation and ribosome recycling. We present that vaccinia an infection enhances uS10 ubiquitylation, indicating an elevated burden on RQC pathways throughout viral propagation.

According to an elevated RQC demand, we exhibit that vaccinia virus replication is impaired in cells that both lack ZNF598 or categorical a ubiquitylation-deficient model of uS10. Utilizing SILAC-based proteomics and concurrent RNA-seq evaluation, we decide that translation, however not transcription of vaccinia virus mRNAs is compromised in cells with poor RQC exercise. Moreover, vaccinia virus an infection reduces mobile RQC exercise, suggesting that co-option of ZNF598 by vaccinia virus performs a crucial function in translational reprogramming that’s wanted for optimum viral propagation.

Guzy jąder zarodkowych powstają przy braku różnicowania specyficznego dla płci 

In response to indicators from the embryonic testis, the germ cell intrinsic issue NANOS2 coordinates a transcriptional program crucial for the differentiation of pluripotent-like primordial germ cells towards a unipotent spermatogonial stem cell destiny. Rising proof signifies that genetic danger components contribute to testicular germ cell tumor initiation by disrupting sex-specific differentiation. Right here, utilizing the 129.MOLF-Chr19 mouse mannequin of testicular teratomas and a NANOS2 reporter allele, we report that the developmental phenotypes required for tumorigenesis, together with failure to enter mitotic arrest, retention of pluripotency and delayed sex-specific differentiation, have been unique to a subpopulation of germ cells failing to specific NANOS2. Single-cell RNA sequencing revealed that embryonic day 15.5 NANOS2-deficient germ cells and embryonal carcinoma cells developed a transcriptional profile enriched for MYC signaling, NODAL signaling and primed pluripotency.

Furthermore, lineage-tracing experiments demonstrated that embryonal carcinoma cells arose completely from germ cells failing to specific NANOS2. Our outcomes point out that NANOS2 is the nexus via which a number of genetic danger components affect tumor susceptibility. We suggest that, within the absence of intercourse specification, indicators native to the growing testis drive germ cell transformation.

Indukowane przez BMP4 różnicowanie komórek macierzystych grzebienia mieszków włosowych ludzkiego włosa w melanocyty prekursorowe z wybrzuszenia mieszków włosowych 

Background: Vitiligo is a pores and skin depigmentation dysfunction, for which, repigmentation remedy with mixed follicular unit extraction (FUE) graft and narrowband ultraviolet B (NBUVB) is taken into account superior to micro-punch graft remedy. BMP4 can induce MITF expression in Neural crest stem cells (NCSCs), and α-MSH subsequently promotes the differentiation of MITF-expressing cells alongside the melanocyte lineage.

Goal: To analyze why FUE grafting is superior to epidermal mini grafting in selling hair follicles (HF) melanocyte cell survival and longevity, we deliberate the in vitro experiments HF bulge NCSCs differentiate into melanocyte precursors underneath the co-treatment of BMP4 and α-MSH.

Strategies: Cells that migrated from the HF bulge of scalp have been cultured and assessed utilizing immunofluorescence. Transcriptome evaluation was carried out on RNA sequencing outcomes.

Outcomes: Fundamental fibroblast progress issue promotes the proliferation and survival of NCSCs, with spontaneous differentiation into SOX10+/SOX2+ glial progenitors, however not into SOX10+/MITF+ precursor melanocytes. Each BMP4 and α-MSH promoted the differentiation into MITF-expressing cells. RNA sequencing revealed a downregulation in neuregulin-1 (NRG1) and sermaphorin 3C (SEMA3C), and upregulation in WNT10A. Moreover, FUE grafting had a supply of reservoir melanocytes superior to mini- grafting in remedy for vitiligo.

Conclusion: We obtained SOX10+/MITF+ precursor melanocytes via an induction of differentiation alongside the melanocyte lineage by BMP4 and α-MSH. Based on the RNA sequencing outcomes that NRG1 and SEMA3C have been downregulated and WNT10A was upregulated, we postulated that HF NCSCs differentiated into melanocyte by co-treatment of BMP4 and α-MSH. Total, FUE grafting is a extra sturdy and substitutive remedy possibility for vitiligo.

Analiza sieci ważonej koekspresji genów genów centralnych w gruczolakoraku płuc 

Lung adenocarcinoma (LUAD) is a tumor with excessive incidence. This examine aimed to determine the central genes of LUAD. LUAD have been analyzed by weighted gene co-expression community (WGCNA), and differentially expressed genes (DEGs) have been recognized. Samples have been obtained from The Most cancers Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases and included 515 LUAD samples and 347 regular samples. The WGCNA algorithm generated a complete of 10 modules. The highest 2 modules (MEturquoise and MEblue) with the best correlation to LUAD have been chosen.

Ten Hub genes (IL6, CDH1, PECAM1, SPP1, THBS1, HGF, SNCA, CDH5, CAV1, and DLC1) have been screened within the intersecting genes of DEGs and WGCNA (MEturquoise and MEblue). Solely SPP1 was correlated with LUAD poor survival, indicating that SPP1 could also be a key Hub gene for LUAD. The competing endogenous RNA (ceRNA) community was constructed to investigate the regulatory relationship of Hub genes, and SPP1 could also be instantly regulated by four microRNAs (miRNAs) and not directly regulated by 49 lengthy noncoding RNAs (lncRNAs).

Leave a Reply

Your email address will not be published. Required fields are marked *