
ILF3-AS1 promuje tlenową glikolizę
Pawel
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ILF3-AS1 promuje tlenową glikolizę i proliferację komórek czerniaka poprzez regulację szlaku miR-493-5p / PDK1
Background: To analyze the function of ILF3-AS1 in regulating the survival of melanoma and its molecular mechanism.
Strategies: The relative expression stage of ILF3-AS1 in melanoma was assessed by qPCR. The impact of ILF3-AS1 and PDK1 on the cell viability was examined by MTT assay. Glucose uptake colorimetric assay, lactate assay, the measurements of extracellular acidification charge (ECAR) and Oxygen consumption charge (OCR) have been carried out to check the impact of ILF3-AS1 and PDK1 on the mobile glycolysis. Luciferase assay was performed to detect the interactions of ILF3-AS1, miR-493-5p and PDK1. RNA immunoprecipitation chip (RIP) assay was used to detect the enrichments of ILF3-AS1 and miR-493-5p within the complicated. Protein stage of PDK1 was detected by western blot evaluation.
Outcomes: qPCR revealed that ILF3-AS1 was upregulated in human melanoma cell strains. MTT assay confirmed that ILF3-AS1 knockdown blunted cell proliferation, which was rescued by the overexpression of PDK1. Glucose uptake colorimetric assay, lactate assay, the measurements of ECAR and OCR indicated that ILF3-AS1 promoted glycolysis via PDK1. Western blotting outcomes confirmed that ILF3-AS1 overexpression promoted PDK1 expression, which was prevented by miR-493-5p overexpression in SK-MEL-1 cells.
Conclusions: ILF3-AS1 promotes the cardio glycolysis and survival of melanoma cells involving miR-493-5p/PDK1 pathway.

Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx126458 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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abx011911-100ul | Abbexa | 100 ul | EUR 493.2 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx032759-400ul | Abbexa | 400 ul | EUR 627.6 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx032759-80l | Abbexa | 80 µl | EUR 343.2 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx034004-400ul | Abbexa | 400 ul | EUR 627.6 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx034004-80l | Abbexa | 80 µl | EUR 343.2 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx037361-100ug | Abbexa | 100 ug | EUR 469.2 |
Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx320518 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx329342 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx329620 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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abx331681-100ul | Abbexa | 100 ul | EUR 510 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx332050-100ul | Abbexa | 100 ul | EUR 510 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx332098-100ul | Abbexa | 100 ul | EUR 510 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx332214-100ul | Abbexa | 100 ul | EUR 510 |
Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx326789 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx326790 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx326791 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx328550 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx328752 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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20-abx214314 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Antibody |
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abx237141-100ug | Abbexa | 100 ug | EUR 610.8 |
Retinoblastoma Protein 1 (RB1) Antibody |
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abx237142-100ug | Abbexa | 100 ug | EUR 661.2 |
Retinoblastoma Antibody / Rb1 |
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RQ5140 | NSJ Bioreagents | 100ul | EUR 419 |
Retinoblastoma Antibody / Rb1 |
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RQ5141 | NSJ Bioreagents | 100ul | EUR 419 |
Retinoblastoma Antibody / Rb1 |
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F54327-0.05ML | NSJ Bioreagents | 0.05 ml | EUR 165 |
Retinoblastoma Antibody / Rb1 |
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F54327-0.2ML | NSJ Bioreagents | 0.2 ml | EUR 379 |
Retinoblastoma Antibody / Rb1 |
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V3218-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. |
Retinoblastoma Antibody / Rb1 |
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V3218-20UG | NSJ Bioreagents | 20 ug | EUR 219 |
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. |
Retinoblastoma Antibody / Rb1 |
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V3218SAF-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: The retinoblastoma protein (protein name abbreviated pRb; gene name abbreviated RB or RB1) is a tumor suppressor protein that is dysfunctional in several major cancers. One function of pRb is to prevent excessive cell growth by inhibiting cell cycle progression until a cell is ready to divide. When the cell is ready to divide, pRb is phosphorylated, becomes inactive and allows cell cycle progression. It is also a recruiter of several chromatin remodeling enzymes such as methylases and acetylases. Rb restricts the cell's ability to replicate DNA by preventing its progression from the G1 (first gap phase) to S (synthesis phase) phase of the cell division cycle. Rb binds and inhibits transcription factors of the E2F family, which are composed of dimers of an E2F protein and a dimerization partner (DP) protein. The transcription activating complexes of E2 promoter-binding–protein-dimerization partners (E2F-DP) can push a cell into S phase. As long as E2F-DP is inactivated, the cell remains stalled in the G1 phase. When Rb is bound to E2F, the complex acts as a growth suppressor and prevents progression through the cell cycle. The Rb-E2F/DP complex also attracts a histone deacetylase (HDAC) protein to the chromatin, reducing transcription of S phase promoting factors, further suppressing DNA synthesis.[Wiki] mAb 1F8 ecognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. |
Retinoblastoma Antibody / Rb1 |
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V3438-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3438-20UG | NSJ Bioreagents | 20 ug | EUR 219 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3438SAF-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3439-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3439-20UG | NSJ Bioreagents | 20 ug | EUR 219 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3439SAF-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3578-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3578-20UG | NSJ Bioreagents | 20 ug | EUR 219 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3578IHC-7ML | NSJ Bioreagents | 7 ml | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Antibody / Rb1 |
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V3578SAF-100UG | NSJ Bioreagents | 100 ug | EUR 499 |
Description: Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma. |
Retinoblastoma Protein 1 (RB1) Protein |
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20-abx260454 | Abbexa |
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Recombinant Retinoblastoma Protein 1 (RB1) |
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4-RPB211Hu01 | Cloud-Clone |
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Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli |
Recombinant Retinoblastoma Protein 1 (RB1) |
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4-RPB211Hu02 | Cloud-Clone |
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Description: Recombinant Human Retinoblastoma Protein 1 expressed in: E.coli |
Retinoblastoma Protein 1 (Rb1) Antibody Pair |
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abx117561-1pair5x96wellplates | Abbexa | 1 pair (5x96 well plates) | EUR 1212 |
Retinoblastoma Protein 1 (RB1) Antibody (Biotin) |
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20-abx272104 | Abbexa |
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Rat Retinoblastoma-associated protein (Rb1) |
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1-CSB-RP155294r(A3) | Cusabio |
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Description: Recombinant Rat Retinoblastoma-associated protein(Rb1),partial expressed in E.coli |
Human Retinoblastoma Protein 1 (RB1) Protein |
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20-abx068874 | Abbexa |
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Human Retinoblastoma Protein 1 (RB1) Protein |
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20-abx068875 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human) |
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4-PAB211Hu01 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1) |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human) |
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4-PAB211Hu02 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1) |
Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody |
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20-abx125454 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser612 (RB1 pS612) Antibody |
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20-abx323083 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody |
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abx333137-100ul | Abbexa | 100 ul | EUR 560.4 |
Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody |
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abx333182-100ul | Abbexa | 100 ul | EUR 560.4 |
Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody |
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abx333183-100ul | Abbexa | 100 ul | EUR 560.4 |
Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody |
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abx333270-100ul | Abbexa | 100 ul | EUR 560.4 |
Retinoblastoma Protein 1 Phospho-Ser780 (RB1 pS780) Antibody |
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20-abx329208 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser811 (RB1 pS811) Antibody |
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20-abx329209 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Thr826 (RB1 pT826) Antibody |
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20-abx329210 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser249 (RB1 pS249) Antibody |
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20-abx326387 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser788 (RB1 pS788) Antibody |
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20-abx326427 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Thr821 (RB1 pT821) Antibody |
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20-abx326768 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser795 (RB1 pS795) Antibody |
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20-abx328301 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser807 (RB1 pS807) Antibody |
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20-abx328302 | Abbexa |
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Retinoblastoma Protein 1 Phospho-Ser608 (RB1 pS608) Antibody |
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20-abx328753 | Abbexa |
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Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC |
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4-PAB211Hu01-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated |
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4-PAB211Hu01-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3 |
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4-PAB211Hu01-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC |
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4-PAB211Hu01-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP |
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4-PAB211Hu01-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE |
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4-PAB211Hu01-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), APC |
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4-PAB211Hu02-APC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with APC. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Biotinylated |
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4-PAB211Hu02-Biotin | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Biotin. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), Cy3 |
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4-PAB211Hu02-Cy3 | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with Cy3. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), FITC |
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4-PAB211Hu02-FITC | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with FITC. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), HRP |
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4-PAB211Hu02-HRP | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with HRP. |
Retinoblastoma Protein 1 (RB1) Polyclonal Antibody (Human), PE |
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4-PAB211Hu02-PE | Cloud-Clone |
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Description: A Rabbit polyclonal antibody against Human Retinoblastoma Protein 1 (RB1). This antibody is labeled with PE. |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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20-abx152929 | Abbexa |
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Monkey Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx358739-96tests | Abbexa | 96 tests | EUR 990 |
Rabbit Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx363643-96tests | Abbexa | 96 tests | EUR 990 |
Sheep Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx364326-96tests | Abbexa | 96 tests | EUR 1111.2 |
Pig Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx360817-96tests | Abbexa | 96 tests | EUR 990 |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx253095-96tests | Abbexa | 96 tests | EUR 848.4 |
Chicken Retinoblastoma Protein 1 (RB1) ELISA Kit |
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abx355616-96tests | Abbexa | 96 tests | EUR 990 |
Human Retinoblastoma Protein 1 (RB1) CLIA Kit |
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20-abx492490 | Abbexa |
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Human RB1(Retinoblastoma Protein 1) ELISA Kit |
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EH3703 | FN Test | 96T | EUR 628.92 |
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.094 ng/ml |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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SEB211Hu-10x96wellstestplate | Cloud-Clone | 10x96-wells test plate | EUR 5402.92 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids. |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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SEB211Hu-1x48wellstestplate | Cloud-Clone | 1x48-wells test plate | EUR 550.13 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids. |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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SEB211Hu-1x96wellstestplate | Cloud-Clone | 1x96-wells test plate | EUR 734.46 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids. |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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SEB211Hu-5x96wellstestplate | Cloud-Clone | 5x96-wells test plate | EUR 2945.08 |
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Retinoblastoma Protein 1 (RB1) in Tissue homogenates, cell lysates and other biological fluids. |
Human Retinoblastoma Protein 1 (RB1) ELISA Kit |
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4-SEB211Hu | Cloud-Clone |
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Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Retinoblastoma Protein 1 (RB1) in samples from Tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species. |
Rozpoznanie H3K9me1 przez czynnik metylacji DNA SHH2 kierowany przez kukurydzę RNA
RNA-directed DNA methylation (RdDM) is a plant-specific de novo DNA methylation pathway, which has intensive crosstalk with histone modifications. Right here, we report that the maize RdDM regulator SAWADEE HOMEODOMAIN HOMOLOG 2 (SHH2) is an H3K9me1 reader. Our structural research reveal that H3K9me1 recognition is achieved by recognition of the methyl group through a basic fragrant cage and hydrogen-bonding and salt bridge interactions with the free protons of the mono-methyllysine.
The di- and tri-methylation states disrupt the polar interactions, lowering the binding affinity. Our examine reveals a mono-methyllysine recognition mechanism which doubtlessly hyperlinks RdDM to H3K9me1 in maize. This text is protected by copyright. All rights reserved.
Działanie kontrolujące jakość rybosomów nasila syntezę białek wirusa krowianki podczas infekcji
Viral an infection each prompts stress signaling pathways and redistributes ribosomes away from host mRNAs to translate viral mRNAs. The intricacies of this ribosome shuffle from host to viral mRNAs are poorly understood. Right here, we uncover a job for the ribosome-associated high quality management (RQC) issue ZNF598 throughout vaccinia virus mRNA translation. ZNF598 acts on collided ribosomes to ubiquitylate 40S subunit proteins uS10 (RPS20) and eS10 (RPS10), initiating RQC-dependent nascent chain degradation and ribosome recycling. We present that vaccinia an infection enhances uS10 ubiquitylation, indicating an elevated burden on RQC pathways throughout viral propagation.
According to an elevated RQC demand, we exhibit that vaccinia virus replication is impaired in cells that both lack ZNF598 or categorical a ubiquitylation-deficient model of uS10. Utilizing SILAC-based proteomics and concurrent RNA-seq evaluation, we decide that translation, however not transcription of vaccinia virus mRNAs is compromised in cells with poor RQC exercise. Moreover, vaccinia virus an infection reduces mobile RQC exercise, suggesting that co-option of ZNF598 by vaccinia virus performs a crucial function in translational reprogramming that’s wanted for optimum viral propagation.
Guzy jąder zarodkowych powstają przy braku różnicowania specyficznego dla płci
In response to indicators from the embryonic testis, the germ cell intrinsic issue NANOS2 coordinates a transcriptional program crucial for the differentiation of pluripotent-like primordial germ cells towards a unipotent spermatogonial stem cell destiny. Rising proof signifies that genetic danger components contribute to testicular germ cell tumor initiation by disrupting sex-specific differentiation. Right here, utilizing the 129.MOLF-Chr19 mouse mannequin of testicular teratomas and a NANOS2 reporter allele, we report that the developmental phenotypes required for tumorigenesis, together with failure to enter mitotic arrest, retention of pluripotency and delayed sex-specific differentiation, have been unique to a subpopulation of germ cells failing to specific NANOS2. Single-cell RNA sequencing revealed that embryonic day 15.5 NANOS2-deficient germ cells and embryonal carcinoma cells developed a transcriptional profile enriched for MYC signaling, NODAL signaling and primed pluripotency.
Furthermore, lineage-tracing experiments demonstrated that embryonal carcinoma cells arose completely from germ cells failing to specific NANOS2. Our outcomes point out that NANOS2 is the nexus via which a number of genetic danger components affect tumor susceptibility. We suggest that, within the absence of intercourse specification, indicators native to the growing testis drive germ cell transformation.
Indukowane przez BMP4 różnicowanie komórek macierzystych grzebienia mieszków włosowych ludzkiego włosa w melanocyty prekursorowe z wybrzuszenia mieszków włosowych
Background: Vitiligo is a pores and skin depigmentation dysfunction, for which, repigmentation remedy with mixed follicular unit extraction (FUE) graft and narrowband ultraviolet B (NBUVB) is taken into account superior to micro-punch graft remedy. BMP4 can induce MITF expression in Neural crest stem cells (NCSCs), and α-MSH subsequently promotes the differentiation of MITF-expressing cells alongside the melanocyte lineage.
Goal: To analyze why FUE grafting is superior to epidermal mini grafting in selling hair follicles (HF) melanocyte cell survival and longevity, we deliberate the in vitro experiments HF bulge NCSCs differentiate into melanocyte precursors underneath the co-treatment of BMP4 and α-MSH.
Strategies: Cells that migrated from the HF bulge of scalp have been cultured and assessed utilizing immunofluorescence. Transcriptome evaluation was carried out on RNA sequencing outcomes.
Outcomes: Fundamental fibroblast progress issue promotes the proliferation and survival of NCSCs, with spontaneous differentiation into SOX10+/SOX2+ glial progenitors, however not into SOX10+/MITF+ precursor melanocytes. Each BMP4 and α-MSH promoted the differentiation into MITF-expressing cells. RNA sequencing revealed a downregulation in neuregulin-1 (NRG1) and sermaphorin 3C (SEMA3C), and upregulation in WNT10A. Moreover, FUE grafting had a supply of reservoir melanocytes superior to mini- grafting in remedy for vitiligo.
Conclusion: We obtained SOX10+/MITF+ precursor melanocytes via an induction of differentiation alongside the melanocyte lineage by BMP4 and α-MSH. Based on the RNA sequencing outcomes that NRG1 and SEMA3C have been downregulated and WNT10A was upregulated, we postulated that HF NCSCs differentiated into melanocyte by co-treatment of BMP4 and α-MSH. Total, FUE grafting is a extra sturdy and substitutive remedy possibility for vitiligo.
Analiza sieci ważonej koekspresji genów genów centralnych w gruczolakoraku płuc
Lung adenocarcinoma (LUAD) is a tumor with excessive incidence. This examine aimed to determine the central genes of LUAD. LUAD have been analyzed by weighted gene co-expression community (WGCNA), and differentially expressed genes (DEGs) have been recognized. Samples have been obtained from The Most cancers Genome Atlas (TCGA) and Genotype Tissue Expression (GTEx) databases and included 515 LUAD samples and 347 regular samples. The WGCNA algorithm generated a complete of 10 modules. The highest 2 modules (MEturquoise and MEblue) with the best correlation to LUAD have been chosen.
Ten Hub genes (IL6, CDH1, PECAM1, SPP1, THBS1, HGF, SNCA, CDH5, CAV1, and DLC1) have been screened within the intersecting genes of DEGs and WGCNA (MEturquoise and MEblue). Solely SPP1 was correlated with LUAD poor survival, indicating that SPP1 could also be a key Hub gene for LUAD. The competing endogenous RNA (ceRNA) community was constructed to investigate the regulatory relationship of Hub genes, and SPP1 could also be instantly regulated by four microRNAs (miRNAs) and not directly regulated by 49 lengthy noncoding RNAs (lncRNAs).